Fig. 2.

The edFAS is deficient in FAS-mediated apoptosis. A). Similar levels of edFAS and wtFAS were expressed on 293 cell surface as detected by the anti-FAS mAb. B). Intracellular flow cytometry assay shows that anti-edFAS mAb (1D4) reacted with edFAS but not with wtFAS expressed in 293 cells. C). The edFAS protein expressed on 293 cells (293 edFAS) could also be detected by anti edFAS mAb (1D4) in a Western blotting assay. No edFAS was detected in 293 cells expressing the wtFAS (293 wtFAS). D). Defective FAS-mediated cell death in 293 cells expressing edFAS. Cell viability was determined with ATPLite Luminescent ATP detection assay. Significant cell death was observed in 293 cells expressing wild-type FAS (wtFAS) after cells were treated with SuperFAS ligand (20 ng/ml) for 4, 6 and 18 hours. No significant cell death occurred in 293 cells expressing edFAS with the same treatment. All experiments have been repeated three times.