Table 1.
Distribution of mutant FAS cDNA clones in SLE patients and normal controls
| Donor | cDNA | Genomic DNA | |||
|---|---|---|---|---|---|
| Total clones | Normal clones | Mutant (+A) clones | Frequency of +A clone (%) | +A clone (total clone) | |
| SLE patient* | 244 | 216 | 28 | 11 | 0 (180) |
| SLE07 | 35 | 30 | 5 | 14 | 0 (36) |
| SLE15 | 36 | 36 | 0 | 0 | - |
| SLE22 | 35 | 31 | 4 | 12 | 0 (36) |
| SLE23 | 35 | 34 | 1 | 3 | - |
| SLE24 | 33 | 30 | 3 | 9 | 0 (36) |
| SLE26 | 35 | 26 | 9 | 26 | 0 (36) |
| SLE27 | 35 | 29 | 6 | 17 | 0 (36) |
|
| |||||
| Controls | 231 | 229 | 2 | 1 | 0 (72) |
| CNTL05 | 32 | 32 | 0 | 0 | 0 (36) |
| CNTL08 | 32 | 32 | 0 | 0 | - |
| CNTL12 | 32 | 32 | 0 | 0 | - |
| CNTL20 | 35 | 33 | 2 | 6 | 0 (36) |
| CNTL29 | 33 | 33 | 0 | 0 | - |
| CNTL34 | 33 | 33 | 0 | 0 | - |
| CNTL71 | 34 | 34 | 0 | 0 | - |
*
The mutant FAS cDNA clones (clones with adenine insertion mutation) was significantly increased in SLE patient group than in the non-SLE controls (2 × 2 contingency table, χ2 = 20.82, p < 0.001, Odds ratio = 14.84).