Figure 4.

CIB1-induced cell migration is inhibited by PI-3 kinase inhibitor.

A: Western blot analysis of cell lysates from mock or CIB1-overexpressing cells treated with DMSO or with 10 nM wortmannin (Wort). Immunoblots were incubated with phospho-specific anti-Erk1/2 (p-Erk1/2) and the blots were reprobed for total Erk1/2. Shown is a representative blot from three separate experiments. B: Confocal images of DMSO or 10 nM wortmannin treated cells spread on Fn (scale bar 10 μm). C: Quantitative analysis of transwell migration assay of mock or CIB1-overexpressing cells untreated or treated with various concentrations of wortmannin as indicated were allowed to migrate on Fn for 5 h. Triplicate inserts were used for each treatment. Ten random views of each insert were counted. Data is expressed as percent control. Number of mock cells migrating across the membrane was considered 100 percent. Each experiment was repeated independently three times. *P<0.05 vs untreated CIB1 overexpressed.