Figure 5.

Src family kinase inhibitors, but not genistein, affects CIB1-induced cell migration. A: Western blot analysis of lysates from mock or CIB1-overexpressing cells treated with DMSO or with 10 μM genistein or 1 μM PP2. Immunoblots were incubated with phospho-specific anti-Erk1/2 (p-Erk1/2) and the blots were reprobed for total Erk1/2. Shown is a representative blot from three separate experiments. B: Confocal images of DMSO or 10 μM genistein treated cells. Cells were stained for phospho-tyrosine (PY20) and CIB1. C: Quantitative analysis of transwell migration assay of mock or CIB1-overexpressing cells untreated or treated with various concentrations of genistein as indicated. D: Confocal images of DMSO or 10 μM herbimycin treated cells. Cells were stained for F-actin and CIB1. E&F: Quantitative analysis of transwell migration assay of mock or CIB1-overexpressing cells untreated or treated with various concentrations of inhibitors as indicated. Three separate inserts for each treatment were used. Ten random views of each insert were counted. Data is expressed as percent control. Number of mock cells migrating across the membrane was considered 100 percent. Each experiment was repeated three times. Scale bar 10 μm.