Table 1.
RT primer design for steroidogenic enzymes.
| Gene name | GenBank accession ID | Nucleotide sequences (5′ – 3′) |
|---|---|---|
| STS | XM_001088752 | Forward AGGACAGGATCATTGATGGACG |
| Reverse TGGCAAAGCATCCATTGGA | ||
| SULT2B1 | XM_001111839 | Forward CGCCCAGCTAATTTGTGTCCT |
| Reverse TCAGAGCCTTGGTCCCTCTTCT | ||
| 3BHSD1/2 (v.1–3) | XM_001113873 | Forward CCACACGGTGACATTGTCAAAT |
| Reverse CCCACATGCACATCTCTGTCAT | ||
| 17BHSD1 | NM_001047132 | Forward GACCCATCCCAGAGCTTCAAA |
| Reverse TGCGTTACACACCAGCACGT | ||
| 17BHSD2 | XM_001111794 | Forward TCACATAACTCAGGCTGCCTCC |
| Reverse CCATCCAGTTCCACAGCTGC | ||
| 17BHSD3 (v.2) | XM_001105829 | Forward AGGCCCTGCAAGAGGAATATAGAG |
| Reverse CCTGACCTTGGTGTTGAGCTTC | ||
| 17BHSD4 (v.1,3) | XM_001087837 | Forward GTGGATCTTGCACCAACATCTGG |
| Reverse CTCTGGCCTTCAGCCTGCCAC | ||
| 17BHSD5 (v.1,2) | XM_001104543 | Forward GAAGTAAAGCTTTGGAGGTCT |
| Reverse CCATCGTTTGTCTCGCTGAGA | ||
| AROMATASE | XM_001082665 | Forward CCAGCAGACCCAGGACTCTAAA |
| Reverse CCAGGACCTGGTATTGAGGATG |
Reference sequences for the targeted Macaca mulatta genes can be accessed via GenBank accession ID