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. 2011 Mar 8;21(2):331–342. doi: 10.1089/scd.2010.0587

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 1. — Vascular development in Flk 1^GFP/+ and wild-type ESC-derived EBs. (A) Flk 1^GFP/+ EBs on day 6 (a), day 9 (b), and day 11 (c). (B) Immunofluorescence staining for Flk 1 in wild-type EBs on day 6 (d), day 9 (e), and day 11 (f). (C) Flow cytometry performed on day 11 Flk 1^GFP/+ EB cells. (D) Fluorescence-activated cell sorting analysis of the percentage of Flk 1⁺ cells in both the Flk 1^GFP/+ EBs and wild-type EBs. Scale bar: 200 μm. Flk 1, fetal liver kinase 1; ESC, embryonic stem cell; EBs, embryonic bodies.