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Expression profiles of EC-specific genes in purified Flk 1+ cells over time. Expression levels of Flk 1, Cd 31, VE-cadherin, vWf, Scl/Tal 1, and Oct 4 were detected using reverse transcription–polymerase chain reaction on the undifferentiated ESCs (ES) and Flk 1+ cells derived from day 4 (d 4), 6 (d 6), 8 (d 8), 11 (d 11), and 13 (d 13) EBs. GAPDH mRNA provided a positive control for RNA quantity. EC, endothelial cell.
