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. 2011 Mar 8;21(2):331–342. doi: 10.1089/scd.2010.0587

FIG. 5.

FIG. 5.

Formation of the capillary network by ESC-ECs in aggregates embedded in the 3D collagen gel sandwich. (A) Aggregates seeded in the 3D collagen gel sandwich for 12 h. (B) ESC-ECs migrate out of the aggregates and form typical cord-like structures after 48 h. (C) Cord-like ECs coalesce into a primary vascular plexus on day 3. (D) The remodeling of the primary vascular plexus into a capillary network on day 4. (E) High-power magnification (100×) of the white rectangular area in D. Arrowhead indicates a representative capillary-like structure containing a lumen (asterisk) lined by 5–6 ECs (arrows). One of the lining ECs has abluminal filopodial extensions (twin arrows). (F) Collapse of the capillary network on day 7. Scale bars, 200 μm. 3D, 3-dimensional.