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. Author manuscript; available in PMC: 2011 Oct 19.
Published in final edited form as: Eur J Pharmacol. 2011 Jul 5;668(1-2):57–64. doi: 10.1016/j.ejphar.2011.06.034

Fig. 5.

Fig. 5

Effect of osthole on IGABA in the presence of flumazenil and diazepam in oocytes expressing α1β2γ2S GABAA receptors. Statistical significance (one-way ANOVA, P<0.05) is indicated with (*), n.s.= not significant (P>0.05). (A) Stimulation of IGABA by osthole in the presence of flumazenil (1 μM).The left bar shows the positive allosteric modulation of the GABA (EC5–10)-induced chloride currents by 15 μM osthole. The right bar illustrates that flumazenil (1 μM) does not antagonize the osthole-induced enhancement of IGABA. (B) Representative currents through α1β2γ2S receptors in the absence and presence of the indicated concentrations of osthole or osthole and flumazenil, respectively. The leftmost current represents the GABA control current (10 μM, single bar). (C) Additive effects of osthole and diazepam on IGABA. The left bar illustrates the enhancement of IGABA by 15 μM osthole, the middle bar by 300 nM diazepam, and the right bar illustrates the enhancement of IGABA by co-application of osthole (15 μM) and diazepam. (300 nM). (D) Representative currents through α1β2γ2S receptors in the absence and presence of the indicated concentrations of osthole, diazepam, or osthole and diazepam, respectively. The leftmost current represents the GABA control current (10 μM, single bar).