. Author manuscript; available in PMC: 2012 Dec 15.

Published in final edited form as: J Comp Neurol. 2011 Dec 15;519(18):3684–3699. doi: 10.1002/cne.22681

TABLE 1.

Antibodies Used in This Study

Antigen	Immunogen	Host isotype	Working dilution	Source, cat. no.
Ptch	N-terminal peptide of human Ptch (amino acids 1–40)	Rabbit serum Rabbit serum	1:200 for blots 1:100 for labeling	Abcam, ab53715
Smo	Peptide of human Smo (amino acids 772–787)		1:200 for blots 1:100 for labeling	Abcam, ab38686
β-Actin	N-terminal peptide of the β-isoform of actin (DDDIAALVIDNGSGK)	Mouse IgG₁	1:5,000	Sigma (clone AC-15), A5441
Neurofilament	The nonphosphorylated neurofilament H in homogenized rat brain	Mouse	1:1,000	Covance, SMI-312R
(Smi312)		IgG₁
MAP2	A peptide shared by all isoforms of MAP2 (KNVRSKVGSTENIKHQPGGGRAK)	Mouse IgG₁	1:2,500	Sigma (clone HM-2), M9942
Bassoon	A peptide of rat bassoon (amino acids 738–1,035)	mouse IgG_2a	1:100	Stressgen, VAM-PS003
Adenylyl cyclase III (ACIII)	C-terminal peptide of mouse ACIII(PAAFPNGSSVTLPHQVVDNP)	Rabbit serum	1:500	Santa Cruz, Sc-588