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. 2011 Oct 15;124(20):3399–3404. doi: 10.1242/jcs.086710

Fig. 3.

Fig. 3.

miR-21 modulates the effects of BMP4 on gene expression, cell proliferation and migration in keratinocytes. (A–E) qRT-PCR analysis of gene expression in the primary mouse keratinocytes relatively to Gapdh levels. (A) BMP4 treatment causes a significant increase in the expression of Id1, Id2, Id3 and Msx2 compared with untreated cells. (B) Upregulation in the expression of Pdcd4, Pten, Timp3 and Tpm1 transcripts by BMP4 treatment. (C) Expression of Id1, Id2, Id3 and Msx2 is not affected by transfection with miR-21 mimic (pro-miR-21). (D) Expression of Pdcd4, Pten, Timp3 and Tpm1 is decreased after treatment with miR-21 mimic. (E) miR-21 mimic prevents BMP4-induced expression of Pdcd4, Pten, Timp3 and Tpm1 in keratinocytes. (F) Expression of Pten, Pdcd4, Timp3 and Tpm1 in the skin of K14-noggin transgenic mice is decreased compared with wild-type mice. (G) Flow cytometry analysis of the cell cycle in primary keratinocytes: a significant decrease in number of proliferating cells caused by BMP-4 treatment was prevented by the miR-21 mimic. (H) Cell migration (scratch) assay. miR-21 mimic significantly increases HaCaT cell migration compared with the control, and interferes with the suppression of cell migration induced by BMP4; *P<0.01, **P<0.001.