Table I.
Time frame for a cycle with PB biopsy and array CGH.
| Bonn |
Bologna |
|||
|---|---|---|---|---|
| Oocyte retrieval | Day 0 | Approx. 13:00 | Day 0 | Approx. 8:00 |
| ICSI | Day 0 | 20:30–21:00 | Day 0 | 11:00–11:30 |
| Biopsy/transfer to PCR tubes | Day 1 | 5:20–5:40 | Day 0 | 20:00–20:30a |
| Amplification | Day 1 | 5:45–9:00 | Day 0 | 20:40–23:45 |
| Labelling/concentration | Day 1 | 9:00–12:00 | Day 0–Day 1 | 23:45–03:25 |
| Hybridization | Day 1 | 12:00–15:15 | Day 1 | 3:25–6:30 |
| Washing: | Day 1 | 15:15–15:55 | Day 1 | 6:30–7:05 |
| Scanning/diagnosisb | Day 1 | 16:05–17:05 | Day 1 | 7:05–8:05 |
| Oocyte selection | Day 1 | 17:05 | Day 1 | 8:10 |
aThis is the time adopted for the last 65 consecutive biopsies in Bologna. For the previous oocytes, biopsy was performed 6–7 h after ICSI.
bTime frame for scan and diagnosis for six oocytes corresponding to 12 PBs. Additional six oocytes will require another 70 min.