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. 2011 Aug 11;20(22):4299–4310. doi: 10.1093/hmg/ddr356

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Figure 4. — DNA methylation directly silences LAMB3 and RUNX3 P1. (A) Luciferase activities of methylated and unmethylated pCpGL-LAMB3 in HaCaT and 623 melanoma cells. M.SssI with the presence of SAM methylates all CpG sites. (B) Luciferase activities of methylated and unmethylated pCpGL-RUNX3 P1 in HaCaT and 623 melanoma cells. Bars are the average of technical triplicates + the standard deviation. Same trend was observed in three biological experiments. Stars represent HpaII sites and asterisks represent HhaI sites.