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. 2011 Aug 16;20(22):4345–4359. doi: 10.1093/hmg/ddr362

Figure 1.

Figure 1.

Generation of MMP-9G100L Tg mice. (A) Design of the construct used for generation of MMP-9G100L Tg mice. (B). C2C12 myoblasts plated in six-well tissue culture plate were transfected with vector alone, WT MMP-9 or indicated amounts of pCCLMCKII-MMP-9G100L plasmid. Cells were differentiated into myotubes and levels of MMP-9 in culture supernatants were measured by gelatin zymography and western blot (WB). Representative photomicrographs of zymography and immunoblot gels are presented here. (C) Agarose gel run after polymerase chain reaction (PCR) showing presence of transgene in founder mouse # 1, 2, 3 and 9. (D) TA muscle extracts prepared from two independent colonies (lines #1 and 9) of MMP-9G100L Tg mice and littermate WT mice were analyzed by zymography and western blotting. Data presented here show expression of active MMP-9 protein in muscle of MMP-9G100L Tg mice. (E) Double immunostaining of muscle sections prepared from TA muscle of 3 months old of WT and MMP-9G100L Tg mice using anti-MMP-9 and anti-laminin. Scale bar: 20 µm.