Table 2.
Antibacterial and antifungal activity of the essential oil of M. vulgare using agar disc diffusion, IC50 and minimal inhibition concentration (MIC).
| Strains | DDa | IC50b | MICc | DDd |
|---|---|---|---|---|
| Bacterial strains Gram (+) | ||||
| Staphylococcus aureus | 12.0 ± 0.5 | 2500 ± 50 | >1100.00 | 20 ± 0.5 |
| Staphylococcus epidermidis | 25.2 ± 0.3 | 2200 ± 50 | >590.00 | 26 ± 0.5 |
| Micrococcus luteus | 12.0 ± 0.5 | 1120 ± 50 | >560.00 | 20 ± 1.5 |
| Enterococcus faecalis | 9.6 ± 1.0 | 1140 ± 10 | >680.00 | 25 ± 1.0 |
| Enterobacter cloacae | 13.8 ± 0.6 | 1500 ± 90 | >670.00 | 21 ± 1.4 |
| Staphylococcus aureus 25923 | 18 ± 1.0 | 2600 ± 80 | >1150.00 | 24 ± 0.5 |
| Bacillus subtilis | 13.2 ± 1.0 | 2130 ± 50 | >890.00 | 26 ± 0.6 |
| Bacillus cereus | 6.6 ± 0.4 | 2113 ± 40 | >950.00 | 21 ± 1.0 |
| Bacterial strains Gram (-) | ||||
| Botrytis cinerea | NS | NS | NS | 21 ± 0.5 |
| Pseudomonas aeruginosa 27853 | NS | NS | NS | 20 ± 1.0 |
| Klebsielle pneumoniae WHO24 | NS | NS | NS | 21 ± 0.9 |
| Escherchia coli 25922 | NS | NS | NS | 22 ± 0.8 |
| Salmonella | NS | NS | NS | 29 ± 1.0 |
| Fungal strains | ||||
| Botrytis cinerea | 12.6 ± 0.5 | 2190 ± 12 | >1100.00 | 29 ± 1.0 |
| Fusarium solani | 6.9 ± 0.5 | 3220 ± 20 | >1190.00 | 28 ± 0.6 |
| Penicillium digitatum | 6.6 ± 0.4 | 3200 ± 15 | >1120.00 | 21 ± 0.9 |
| Aspergillus. niger | 6.4 ± 0.0 | 3000 ± 50 | >1180.00 | 30 ± 0.5 |
Results are means of three different experiments,
a DD: Disc Diameter of inhibition (halo size) in (mm), E. oil 100 μg/disc,
b MIC: minimum inhibitory concentration (μg/ml),
cIC50: 50% inhibition concentration (μg/ml),
dDD: Disc Diameter of inhibition zone of ampicillin (10 μg/disc) and cycloheximide (10 μg/disc), were used as positive controls for bacteria and fungi, respectively,
NS: not sensitive.