Figure 4.

Microscope observation of biofilm inhibition. Biofilm inhibition of E. coli PHL628 (A) and Pseudomonas fluorescens (B) on glass cover slip under a phase-contrast microscope at a magnification of 40X. Bacterial cells were incubated with (1) 1X SP1 supernatant, (2) 2 × SP1 concentrated supernatant, (3) 5 × SP1 concentrated supernatant, (4) 10 × SP1 concentrated supernatant. No difference in biofilm production was observed in the presence of 1X, 2X, 5X and 10X M63K₁₀ sterile medium (not shown).