Table 1.
1H, 13C and 31P NMR chemical shift of polysaccharide(p.p.m). Spectra in D2O were measured at 27°C and referenced to internal sodium 3-(trimethylsilyl)-(2,2,3,3-2H4) propionate (δH 0.00), internal methanol (δC 49.00)and to external aq. 85% (v/v) phosphoric acid (δP 0.00)
| Residue | Nucleus | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|---|
| → 4)-α-D-Galp-(1 → | |||||||
| 1H |
5.071 H3Gro (3.7 Hz)a |
3.690 | 3.784 | 3.827C6,4Gal | 3.917C3Gal | 3.671H1Gal | |
| 13C | 99.47H1Gro | 69.37 | 69.95 |
78.32H1Gal (7.8 Hz)b |
70.19 | 62.18H5Gal | |
| Gro-1-P-(O → | |||||||
| 1H | 3.865 C4Gal -3.906 | 4.120C3, 5Gro | 3.839-3.770 | ||||
| 13C |
65.63*H1Gro (4 Hz)a -65.41* (4.5)a |
70.76 H1Gal (7.9 Hz)c |
67.15 (~2 Hz)d |
||||
| 31P | 1.269 | ||||||
*diastereotopic carbons; a 3J H1, H 2; b 2J C-P ; c 3J C-P ; d 4J C-P; in italics, the signals showing C-H long-range correlations with the positions in superscripts; underlined are the NOE contacts with positions in superscripts.