Myocardial expressions of ANP, GATA4, TGF-β1, PKC-α, and OPN. (a) Representative western blots showing specific bands for ANP, GATA4, TGF-β1, PKC-α, OPN, and GAPDH as an internal control. Equal amounts of protein sample (30 μg) obtained from whole ventricular homogenate were applied in each lane. These bands are representative of five separate experiments. (b–f) Densitometric data of protein analysis. The mean density values of ANP, GATA4, TGF-β1, PKC-α, and OPN were expressed as ratios relative to that of GAPDH. Each bar represents mean ± S.E.M. Group control (C), age-matched normal rats; group DNR, DNR-treated rats administered with vehicle; group Carv, DNR-treated rats administered with carvedilol (30 mg/kg/day). **P < .01 versus group Control; ##
P < .01 versus group DNR.