| Component | Amount |
| DMEM(DME/low modified) | 500 ml |
| FBS (fetal bovine serum) heat inactivated | 100 ml |
| HEPES 1.0 M | 5ml |
| Vitamin C (dissolved in PBS or DMEM) | 25 mg |
| Proline/Glycine/Alanine 25 mg/25 mg/10 mg (dissolved in 5ml of PBS) | 5ml |
| CuSO4 1.5 μg (dissolved in1 ml of PBS) | 1ml |
| Penicillin G at 10,000 units/ml | 5ml |
| PDGF-BB (platelet-derived growth factor-BB) at 10ng/ml | 5μg |
| bFGF (basic fibroblast growth factor) at 10ng/ml | 5μg |
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