| A. One T-175 cm2 tissue culture flask (4000 μL/flask): prepare for rRBD expression | |||
| Tube A | Tube B | ||
| 2X HBS | 2000 μL | 2.5M CaCl2 | 200 μL |
| rRBD plasmid DNA | 40 μg | ||
| DiH2O to | 2000 μL | ||
| B. One 100-mm petri dish (1000 μL/dish): prepare for SARS pseudovirus production | |||
| Tube A | Tube B | ||
| 2X HBS | 500 μL | 2.5M CaCl2 | 50 μL |
| SARS-CoV S plasmid DNA | 5 μg | ||
| HIV-1 plasmid (pNL4-3.luc.RE) | 5 μg | ||
| DiH2O to | 500 μL |
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