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. 2011 Oct 17;6(10):e26297. doi: 10.1371/journal.pone.0026297

Figure 3. Both human and rat USP46s have deubiquitinating activity toward the model substrate of GST-Ub52.

Figure 3

(A) Constructs of wild-type (WT) and mutant USP46 (C44S) for the USP cleavage assay. (B) Cleavage of model substrate GST-Ub52 by USP46. The GST-Ub52 was coexpressed with indicated proteins induced by IPTG in E coli BL21. The arrows indicate the GST-Ub52 (about 42 kDa) and its cleavage products GST-Ub (about 36 kDa), which were purified by glutathione-agarose beads and detected by Coomassie Brilliant Blue. UBP2 (a yeast deubiquitinase) and pAC-T7 (an empty plasmid vector) are used as positive and negative controls, respectively.