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. 2011 Oct 17;6(10):e26401. doi: 10.1371/journal.pone.0026401

Figure 3. Downregulation of RPL6 represses gastric cancer cell growth in vitro.

Figure 3

A. Detection of RPL6 in SGC7901/AGS, SGC7901/AGS-siRPL6-1,SGC7901/AGS-siRPL6-2, SGC7901/AGS-siRPL6-Control cells. β-actin was detected as a loading control. B. The growth curves of SGC7901/AGS cell variants with down-regulated RPL6 were determined. The proliferation of SGC7901/AGS cell derivates was monitored with MTT assay as described in “Materials and Methods” and their growth curves were plotted. Error bars correspond to mean ± SD. P<0.05 compared with control cells. C. Colony formation ability of SGC7901/AGS cell variants with down-regulated RPL6 was determined with soft agar colony formation assay as described in “Materials and Methods”. Values represent the mean ± SD of triplicates in each experiment. Shown are representative of at least three separate experiments. (p<0.05).