Table 2. List of oligonucleotides used in this paper.
| Name | Sequence 5′–3′a,b,c |
|---|---|
| Bacterial 5′ (967F) | |
| gcctccctcgcgccatcagNNNNNCNACGCGAAGAACCTTANC | |
| gcctccctcgcgccatcagNNNNNCAACGCGAAAAACCTTACC | |
| gcctccctcgcgccatcagNNNNNATACGCGARGAACCTTACC | |
| gcctccctcgcgccatcagNNNNNCAACGCGCAGAACCTTACC | |
| gcctccctcgcgccatcagNNNNNCTAACCGANGAACCTYACC | |
| Bacterial 3′ (1046R) | |
| gccttgccagcccgctcagCGACAGCCATGCANCACCT | |
| gccttgccagcccgctcagCGACAACCATGCANCACCT | |
| gccttgccagcccgctcagCGACGGCCATGCANCACCT | |
| gccttgccagcccgctcagCGACGACCATGCANCACCT | |
| Archaeal 5′ (958F) | |
| gcctccctcgcgccatcagNNNNNAATTGGANTCAACGCCGG | |
| Archaeal 3′ (1048R) | |
| 1048arcR-major | gccttgccagcccgctcagCGRCGGCCATGCACCWC |
| 1048arcR-minor | gccttgccagcccgctcagCGRCRGCCATGYACCWC |
| B27F | AGAGTTTGATCMTGGCTCAG |
| U1492R | GGTTACCTTGTTACGACTT |
a
Nucleotide sequence presented in lower case is the Roche adaptor sequence A (gcctccctcgcgccatcag) or B (gccttgccagcccgctcag).
b
NNNNN stands for a 5-bp ‘key' used to identify samples in a run containing multiple samples.
c
Nucleotides in upper case are the actual primer sequences, complementary to regions in the 16S rRNA gene.