Table 4. Tentative phylogenetic affiliation of fungal euknr complementary (c)DNA and relative expression levels in microcosms with agricultural soil N (soil from Niederschleinz) after addition of potassium nitrate (KNO₃) and carbohydrates.

		Aa			B			C
	gb	cc	c/gd	g	c	c/g	g	c	c/g
Fusarium sp. 2				6 (2)				69 (25)
Lasiosphaeriaceae sp. 4	3 (1)			3 (1)			3 (1)	3 (1)	1.0
Lasiosphaeriaceae sp. 5	21 (7)	51 (24)	2.4	40 (14)			25 (9)
Trichocladium asperum rel. 1				14 (5)	13 (6)	0.9	11 (4)
Trichocladium asperum rel. 2	21 (7)	49 (23)	2.2	14 (5)	87 (39)	6.1	22 (8)	28 (10)	1.3
Stramenopiles sp.2		- (30)
Ascomycotae	100 (33)	100 (47)		100 (35)	100 (45)		100 (36)	100 (36)

^aTreatments differed in application of organic fertilizer (A: no organic fertilizer; B: 0.05% Biovin; C: 0.05% autoclaved Biovin). For further details on the experimental setup, see Materials and methods.

^bRelative clone abundances (rounded to integer) and absolute clone numbers (in parentheses) in the genomic libraries. Only ascomycetous euknr sequences were considered for calculation of the relative abundances and only species for which euknr cDNA could be found at least in one of the three treatments are shown.

^cRelative clone abundances (rounded to integer) and absolute clone numbers (in parentheses) in the cDNA libraries. Only ascomycetous euknr sequences were considered for calculation of the relative abundances.

^dRatios of relative clone abundances in genomic and cDNA libraries (c/g) were calculated as approximations for expression levels. No relative expression levels were calculated for euknr from Fusarium sp. 2, which was found at the genomic level in treatment B and at the cDNA level in treatment C. Stramenopiles sp. 2 was only found in the cDNA library from treatment A, but in no genomic library.

^eNumber of ascomycetous euknr sequences in the respective libraries, on which the calculation of the relative abundances was based.