Table 2. PCR primers used to screen isolates of Sinorhizobium medicae.

	F primer sequence	R primer sequence	Contig	F location	R location	Length of product
RTXa	AGTCTGTCCTCTACCGTAAG	GTATAAGTGTAGCCGTCCTC	30	3572–3591	4850–4869	1297
RTXb	CTGCCGCAAGCCAATTCAGG	CAATGCCGCGAGCGATCTCT	30	4647–4666	6109–6128	1481
RTXc	GTCCGCCATACATTGATCTC	TCTGCTGTTCGGCTTCATAC	30	5995–6014	7055–7074	1079
REPa	CACACGAACGGCGAAGAATA	ATTCGCTGATTCCGGATGCT	165	855–874	2483–2502	1647
REPb	CATGGAGGTCAGACATTGAG	CCACTACGCCATATTGGCTA	165	2357–2376	2891–2910	553
SIDa	GTGCGCGATGTCAACAACAG	CAGCGGATTCGGCAAGTGAT	211	716–735	2196–2215	1499
SIDb	TCGGCGTTCCAAGCCGTGAT	TACCGCCGAACGGAGATAGC	211	3465–3484	4667–4686	1221
SIDc	GACGCCGGACCAGTAGTCAA	GCGTGGCCTTAACCGTTAGC	211	5157–5176	5613–5632	475
AITa	TCTATGAGCGCATCCACAGG	CGCAAGGATCTGTGACCATT	228	8–27	824–843	724
AITb	GCAATCGGTGTCGTGAATGA	ATCGGCCATCTCGACGTTAT	228	304–323	1427–1446	1142
AITc	AGGAGAACGCGGCGATTGTC	GCCGTCTTCCAAGCCTGCTA	228	716–735	1468–1487	771
AITd	GCTCGATGGACTTCGATGTG	CTCGGTGACGCTGCCGATAA	62, 228	182–201	206–225	542
RKPa	GCGTTGATTCGGCTGCCGAT	AGACCTTCATACGCGACTGG	59	94–113	914–933	839
RKPb	GTATTGCCACGCGAGGACAC	GTTCAGTCGCCGCAGCTATG	59	797–816	1794–1813	1016
RKPc	TCTATCGCCTCGATCAGTCT	TGCGTGTCTTCCATCTCTAC	59	1559–1578	3004–3023	1464
RKPd	ATCGCTCGTGGTAGAGATGG	CGCAAGAACCGTCCTAACAG	59, 179	2994–3013	147–166	989
RKPe	GGAAGTAGTTGCGGCGCTTG	TCCTTCTTGGCGTGTATCGG	179	289–308	994–1013	724
RKPf	GCGGCATTAAGGCGGTGTCT	TCCGGTTAAGCGCATGGTCC	47	593–612	948–967	374
RKPg	TTGACTTCGGCGTGAACTCC	TACGACAGCGATGGCAATGG	47	725–744	2191–2210	1485
RKPh	AATCGGCTGCGGTGATGCTA	GCGGCATTCGGCAATCTTGA	47	2129–2148	3368–3387	1258