Figure 2. Liquid facets-Related is found throughout the ovariole and colocalizes with Golgi, clathrin and some endosome markers.

(A,B) LqfR (red) is expressed in punctae in the germarium (g), in the oocyte (o), nurse cells, and in the surrounding follicle cells during oogenesis in wild type ovaries. LqfR is enriched apically (arrows) in follicle cells beginning around mid-oogenesis as is evident at stage 10B, but is much weaker in the oocyte than in surrounding tissue at this stage. Anterior is to the left and stages are indicated. (C–T) Colocalization of LqfR (red) with various markers (green). (C–E) TGN-localized anti-syntaxin16 [26] in follicle cells from a stage 9 egg chamber. medial-Golgi-localized anti-p120 [45] in migrating border cells (F–H) and in follicle cells from a stage 14 egg chamber (I–K). (L–N) Clathrin light chain-GFP [46], and (O–Q) the early endosome marker Rab5-GFP [47] in follicle cells of stage 9 egg chambers. (R–T) MVP marker anti-Hrs [48] in follicle cells from a stage 10B egg chamber. Some punctae of colocalization are indicated by arrowheads (E,K,N,Q,T). Egg chambers in (C–K, R–T) are from females of genotype hsFLP/+ ; FRT-82B lqfR^D66/FRT-82B ovoD1-18. For (L–Q), expression of the GFP-fusion proteins is driven by TubulinP-Gal4 [49] in a wild type background. Panels on the right (E,H,K,N,Q,T) are merges of the adjacent left two panels and also show To-Pro-3 stained nuclei in blue (except E and H). All images are single confocal slices. Bar, 50 µm (A,B) and 10 µm (C–T).