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. 2011 Oct 17;6(10):e25690. doi: 10.1371/journal.pone.0025690

Figure 3. Multiparameter flow cytometric detection of cytokine expression in CD4+ T-cells of infants at 6 and 12 months of age.

Figure 3

Frequencies of circulating IFN-γ (A), TNF-α (B), and IL-2 (C) secreting cells among CD69+CD4+ T-cells from infants (n = 20) at 6 and 12 months of age after in vitro stimulation with PHA, SEB, PPD, TT, and PMA. The percentages of cytokines expressing cells were measured within lymphocytes electronically gated for CD69+CD3+CD8− cells. Horizontal lines represent the median percentage. The p-values were calculated using the Wilcoxon signed ranks test for paired values.