Skip to main content
. 2011 Oct 17;6(10):e25690. doi: 10.1371/journal.pone.0025690

Figure 4. Multiparameter flow cytometric detection of cytokine expression in CD8+ T-cells of infants at 6 and 12 months of age.

Figure 4

Frequencies of circulating IFN-γ (A), TNF-α (B), and IL-2 (C) secreting cells among CD69+CD8+ T-cells from infants (n = 20) at 6 and 12 months of age after in vitro stimulation with PHA, SEB, PPD, TT, and PMA. The percentages of cytokines expressing cells were measured within lymphocytes electronically gated for CD69+CD3+CD8+ cells. Horizontal lines represent the median percentage. The p-values were calculated using the Wilcoxon signed ranks test for paired values.