Table 1. Statistical comparisons (two-tailed t-test).

		p-value
Question	Comparison	HDMEC	HBMEC	HUVEC
1	RBC/ItG binding vs A4/ItG binding	0.1200	0.0029	0.0007
2	C24/ItG binding vs ItG/ItG binding	0.0004	0.1652	0.1185
3	sbp1 ko/ItG binding vs ItG/ItG binding	0.2072	0.0253	0.4092
4	sbp1 ko/ItG binding vs RBC/ItG binding	0.0030	0.3125	0.0001

1. Does co-culture with IE lead to induction of higher levels of binding compared to RBC co-culture?

2. Are higher levels of binding induced in all three EC by co-culture with ItG (ICAM-1-binding) compared to co-culture with C24 (non ICAM-1-binding)?

3. Do adherent IE (e.g. ItG) induce higher levels of binding on co-culture than the sbp1 ko parasite line?

4. Can co-culture with the sbp1 ko parasite line also induce increased levels of cytoadherence?