Table 2.
Donor position | 581 | 560 | 567 |
---|---|---|---|
Donor lifetime (μs) | 1607 ± 55 | 1607 ± 69 | 1612 ± 38 |
Sensitized emission lifetime (μs) | 81 ± 2 | 77 ± 4 | 380 ± 32 |
Distance (LRET) (Å) | 42 ± 2 | 41 ± 2 | 56 ± 5 |
Distance in crystal structure *(Å) | 44 | 53 | 70 |
Distances are measured between the Ca atoms between the N-terminal I422 and respective donor residues.