(A–C) Double immunostaining of GRPR (red, lamina I) and MOR1D (green, lamina II) revealed no co-localization of GRPR and MOR1 in the dorsal spinal cord.
(D–F) Double immunostaining of GRPR (red) and MOR1D (green) in lamina I of the spinal cord. Arrows indicate co-expression (yellow) and arrowheads indicate singular expression. Cells co-expressing GRPR (11/33) and MOR1D (11/17), which represent ~31% of GRPR-positive cells and ~65% of MOR1D-positive cells respectively, were found in 25 lumbar spinal cord sections.
(G–I) Double immunostaining revealed no co-localization of GRPR (red, lamina I) and MOR1 (green, lamina II) in the dorsal spinal cord.
Scale bar, 50 μm. Also see Figure S2