Fig. 3.
A. aegypti IAP antagonists directly bind to AeIAP1, and binding is dependent on the IBM. a The indicated His-tagged recombinant proteins were incubated with 35S-labeled AeIAP1, after which protein complexes were purified using Talon resin and examined by autoradiography. GB is a control protein, the B1 domain of streptococcal protein G [50]. b Streptavidin-agarose beads were incubated with biotinylated peptides containing amino acids 2–6 of Mx and IMP, or 1–11(Met-Hid) of Hid, or buffer. Recombinant proteins (GST-BIR1, GST-BIR2, or GST) were added, and the protein that bound was eluted and detected by immunoblotting with anti-GST antibody. Input represents 10% of the amount of the relevant protein added to the beads