Fig. 4.

AeIAP1 and DIAP1 inhibit IAP antagonist-induced caspase activation. C6/36 cells (a) or SF-21 cells (b) were co-transfected with IAP antagonist constructs and plasmids expressing AeIAP1 or DIAP1, or a control irrelevant plasmid. Cell lysates were prepared at 23 h post-transfection and caspase activity was determined using Ac-DEVD-AFC as a substrate. Data are shown as mean ± SEM of three independent experiments (***P < 0.0001; *P < 0.01; ^#P < 0.05 by Student’s t test)