Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Apoptosis. 2011 Mar;16(3):235–248. doi: 10.1007/s10495-011-0575-3

Fig. 8.

Fig. 8

IAP antagonists induce caspase activation in mosquito cell cytoplasmic lysate, and cytochrome C does not. a Fifty μg S100 lysate isolated from Aag2 cells was incubated with recombinant protein (Mx or IMP or control GB) (10 μM) prior to determining caspase activity using Ac-IETD-AFC as a substrate. b Fifty μg S100 lysate was incubated with cytochrome C (2 μg), ATP (2 mM) and MgCl2 (1 mM), and caspase activity was determined using Ac-DEVD-AFC as a substrate. Data are shown as mean ± SEM of three independent experiments (***P < 0.0001; NS non-significant by Student’s t test)