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. Author manuscript; available in PMC: 2011 Oct 19.
Published in final edited form as: Environ Mol Mutagen. 2009 Jul;50(6):460–472. doi: 10.1002/em.20482

Fig. 3.

Fig. 3

Dose response for intracellular concentration of WR1065 following treatment of primary mouse (A) or rat (B) lymphocytes for 1 or 2 hr with 0, 10, 50, or 100 μM amifostine converted to WR1065 (n = 2–4/species for each exposure group). Amifostine was dephosphorylated for 30 min with alkaline phosphatase to yield WR1065 for cell treatment. A high-pressure liquid chromatography method with electrochemical detection was used to measure WR1065, and the concentration of WR1065 per million cells in each sample was determined by interpolating the peak areas to a standard curve for WR1065 [Pamujula et al., 2004]. (A) Data from WR1065-exposed mouse lymphocytes; (B) data from WR1065-exposed rat lymphocytes.