TABLE II.
Inhibition of HIV-1 Replication as Assessed by Inhibition of p24 Protein Production in PHA-Stimulated Human T-Cell Blasts by AZT and/or WR1065a
| Exposure groups | p24 protein (pg/ml) | Estimated % inhibition |
|---|---|---|
| Experiment #1 | ||
| Cells (uninfected) | 0.28 | NA |
| HIV-1-infected cells | 498.62 | NA |
| HIV-1-infected cells + 10 μM AZT | 0.13b | ~100% |
| HIV-1-infected cells + 1,000 μM WR1065 | 0.13b | ~100% |
| HIV-1-infected cells + 10 μM AZT + 1,000 μM WR1065 | 0.13b | ~100% |
| Experiment #2 | ||
| Cells alone (uninfected) | 8.99 | NA |
| HIV-1-infected cells | 176.45 | NA |
| HIV-1-infected cells + 10 μM AZT | 9.07b | 94.9% |
| HIV-1-infected cells + 1,000 μM WR1065 | 8.92b | 94.9% |
| HIV-1-infected cells + 330 μM WR1065 | 9.31b | 94.7% |
| HIV-1-infected cells + 100 μM WR1065 | 8.92b | 94.9% |
| Experiment #3 | ||
| Cells alone (uninfected) | 3.0 | NA |
| HIV-1-infected cells | 44,455.8 | NA |
| HIV-1-infected cells + 10 μM AZT | 2.9b | ~100% |
| HIV-1-infected cells + 66 μM WR1065 | 33.2b | 99.9% |
| HIV-1-infected cells + 33 μM WR1065 | 503.2b | 98.9% |
| HIV-1-infected cells + 10 μM WR1065 | 911.2b | 98.0% |
| HIV-1-infected cells + 5 μM WR1065 | 10,293.7b | 76.8% |
NA, not applicable.
a
Primary human lymphocytes were stimulated with PHA, and resulting T-cell blasts were infected with HIV-1BZ-167 for 2 hr, washed, and treated with drug(s) for 72 hr, and then assessed for HIV p24 protein production using an ELISA kit as described in the Materials and Methods.
b
P < 0.001 (χ2 test), compared with p24 production in untreated HIV-infected cells.