. Author manuscript; available in PMC: 2011 Oct 19.

Published in final edited form as: Environ Mol Mutagen. 2009 Jul;50(6):460–472. doi: 10.1002/em.20482

TABLE III.

Impact of WR1065 Treatment on the Replication of Prototype Adenovirus Strains of Serotypes 4, 5, and 7 in A549 Cells^a

Virus	Species	Experiment number	Mean infectious virus yields (PFUs × 10⁶/ml) 96-hr postinfection in the presence of WR1065
Virus	Species	Experiment number	0 μM WR1065	10 μM WR1065	33 μM WR1065	100 μM WR1065
Ad7p (Gomen)	B	1^b	3.5	1.85 (47%)	0.85 (76%)	1.15 (67%)
		2^b	58.2	–	–	30.5 (48%)^e,^d
Ad5p (Adenoid 75)	C	1^b	8.9	6.2 (30%)	5.7 (36%)	2.2 (75%)^e
		2^e	665	–	600 (9%)^e	500 (25%)^f
		3^c	1,920	–	1,660 (14%)^e	–
Ad4p (RI-67)	E	1^b	1.6	0.35 (78%)	–	–
		2^b	22.5	–	–	9.5 (58%)
		3^b	150	–	–	40 (73%)^f
		4^c	1,900	–	1,420 (25%)^e	980 (48%)^f

Infectious virus yields were determined in A549 cells infected with adenovirus and treated with 0 or up to 100 μM WR1065. Cells in six-well plates were infected with a given species of adenovirus. After 1 hr adsorption, cells were replenished with minimum essential medium containing 0 or up to 100 μM WR1065. At 7 days postinfection, infected cells were frozen and infectious virus yields in freeze-thawed preparations were titered by plaque assay.

Infected with 10³ PFUs, adsorption for 1 hr.

Infected with 10⁴ PFUs, adsorption for 1 hr.

Two additional experiments using 100 μM WR1065 yielded similar results.

P < 0.05 (χ² test), compared with untreated infected A549 cells.

P < 0.001 (χ² test), compared with untreated infected A549 cells.