Fig. 1.

AMPK and AMPK regulatory proteins are activated in the aorta of mice exercised to exhaustion on a treadmill. Protein lysates from aortas of sedentary and exercised mice were analyzed by Western blot (A) and quantified in B (a–f). Due to the observation that exercise activates AMPK in fat (27), the peri-aortic fat (PAF; or tunica adventitia) that surrounds the aorta was removed before protein isolation. Thus aortic protein lysates were made only from the tunica media and intima (see Fig. 6B for definitions). To exclude the possibility that the results obtained may have come from incompletely removed PAF, an immunoblot was performed against the fat cell-specific marker aP2 and a positive control of PAF was included in the first lane. As is evident, mouse aortic sample number 13 was contaminated by PAF and was thus excluded from the analysis. SIRT1, silent information regulator-1; ACC, acetyl-CoA carboxylase; p, Phospho; T-, total; AU, arbitrary units; CamK1, Ca²⁺/calmodulin kinase I.