Fig. 6.

Treatment of rats with l-NAME decreases MT1-MMP protein expression in LP mUA. Left: LP and LPLN mUA were dissected and placed in modified RIPA buffer for protein analysis as described in materials and methods. Twenty-five micrograms of protein were loaded on a denaturing gel. Shown is a representative immunoblot. Right: densitometry, as described in materials and methods, was used to quantify the immunoblots probed for MT1-MMP and β-tubulin. MT1-MMP is significantly decreased in mUA of LPLN animals compared with LP. *P < 0.05 by Student's t-test.