Fig. 3.

mRNA expression of the α₂ isoform of the Na,K-ATPase in isolated cardiac myocytes. A: representative qualitative PCR gel from isolated cardiac myocytes using the real-time α₂ and GAPDH primers. B: quantitative real-time PCR analysis of mRNA expression of α₂ in isolated cardiac myocytes normalized to GAPDH expression. Data represent an arbitrary ratio of mRNA expression of α₂ in knockout mice to that expressed in control mice from two independent experiments. Bars represent means ± SE; n = 3. *P = 0.002, statistical difference.