Fig. 1.

Cidea and Ucp-1 are highly expressed in interscapular brown adipose tissue (BAT) and perivascular adipose from the aortic arch (PVAT) independently of obesity. A, B, C, and D: Cidea, Ucp-1, Acrp30, and Pparγ2 expression in normal diet (ND) and high-fat diet (HFD) mice. Quantitative PCR was performed on total RNA isolated from inguinal (SAT), epididymal (VAT), BAT, and PVAT. Expression levels were calculated with the 2^−ΔΔCt method using 36b4 as the reference gene and normalized to expression in SAT from ND conditions. Ucp-1 expression is shown in log10 scale. AU, arbitrary units. ND: n = 12 white; HFD: n = 12, black. Results are means ± SE. ***P < 0.001 vs. SAT ND; ^#P < 0.05. ND vs. HFD of the same fat depot using two-way ANOVA and the Bonferonni correction; P < 0.05 vs. SAT ND.