Table 1.
Depot-specific expression of select gene categories in normal diet conditions
| PVAT vs. SAT |
PVAT vs. VAT |
PVAT vs. BAT |
|||||
|---|---|---|---|---|---|---|---|
| Symbol | Gene Name | FC | P value | FC | P value | FC | P value |
| Most highly upregulated genes | |||||||
| Ucp1 | uncoupling protein 1 | 21.6 | <0.0010 | 31.2 | <0.001 | 1.0 | 0.85 |
| Elovl3 | elongation of very long chain fatty acids (FEN1/Elo2) | 19.3 | <0.001 | 29.3 | <0.001 | 1.1 | 0.54 |
| Slc27a2 | solute carrier family 27 (fatty acid transporter), member 2 | 17.9 | <0.001 | 17.2 | <0.001 | 1.4 | 0.26 |
| Cox7a1 | cytochrome c oxidase, subunit VIIa 1 | 16.1 | <0.001 | 23.1 | <0.001 | 1.0 | 0.61 |
| Cpt1b | carnitine palmitoyltransferase 1b, muscle | 14.5 | <0.001 | 24.7 | <0.001 | 1.0 | 0.44 |
| Kng2 | kininogen 2 | 14.0 | <0.001 | 12.4 | <0.001 | 1.2 | 0.48 |
| Acot11 | acyl-CoA thioesterase 11 | 12.6 | <0.001 | 15.8 | <0.001 | 1.0 | 0.83 |
| Cidea | cell death-inducing DNA fragmentation factor-like effector A | 12.6 | <0.001 | 31.8 | <0.001 | 1.1 | 0.11 |
| Brown adipose-enriched genes | |||||||
| Ucp1 | uncoupling protein 1 (mitochondrial, proton carrier) | 21.6 | <0.001 | 31.2 | <0.001 | 1.0 | 0.85 |
| Elovl3 | elongation of very long chain fatty acids (SUR4/Elo3, yeast)-like 3 | 19.3 | <0.01 | 29.2 | <0.001 | 1.1 | 0.54 |
| Slc27a2 | solute carrier family 27 (fatty acid transporter), member 2 | 17.8 | <0.001 | 17.1 | <0.001 | 1.4 | 0.26 |
| Cox7a1 | cytochrome c oxidase, subunit VIIa 1 | 16.1 | <0.001 | 23.0 | <0.001 | 1.0 | 0.68 |
| Cpt1b | carnitine palmitoyltransferase 1b, muscle | 14.5 | <0.001 | 24.7 | <0.001 | 1.1 | 0.47 |
| Cidea | cell death-inducing DNA fragmentation factor like effector A | 12.6 | <0.001 | 31.7 | <0.001 | 1.1 | 0.11 |
| Dio2 | deiodinase, iodothyronine, type II | 8.0 | <0.001 | 9.9 | <0.001 | −1.1 | 0.68 |
| Prdm16 | PR domain containing 16 | 1.8 | <0.001 | 2.41 | <0.001 | 1.0 | 0.70 |
| White adipose-enriched genes | |||||||
| Hoxc8 | homeobox C8 | −9.7 | <0.001 | −17.7 | <0.001 | 2.1 | 0.05 |
| Apol7c | apolipoprotein L 7c | −8.2 | <0.01 | −1.6 | 0.21 | 1.2 | 0.50 |
| Dapl1 | death associated protein-like 1 | −6.3 | <0.01 | −2.0 | 0.09 | 1.8 | 0.16 |
| Nnat | Neuronatin | −6.1 | <0.01 | −6.9 | <0.01 | 1.1 | 0.79 |
| Sncg | synuclein, gamma | −4.2 | <0.01 | −5.5 | <0.001 | 1.0 | 0.78 |
| Stap1 | signal transducing adaptor family member 1 | −4.7 | <0.05 | −1.4 | 0.48 | 2.9 | 0.07 |
| Grap2 | GRB2-related adaptor protein 2 | −3.9 | <0.01 | −1.4 | 0.22 | 1.5 | 0.15 |
| Mest | mesoderm specific transcript | −3.8 | <0.01 | −14.2 | <0.001 | 1.0 | 0.74 |
| Immune cell-enriched genes | |||||||
| Il7r | interleukin 7 receptor | −5.7 | <0.01 | −1.3 | 0.47 | 2.4 | 0.13 |
| Ccl5 | chemokine (C-C motif) ligand 5 | −5.6 | <0.05 | −1.3 | 0.37 | 1.6 | 0.48 |
| Cd3g | CD3 antigen, gamma polypeptide | −5.0 | <0.01 | −1.7 | 0.21 | 2.3 | 0.06 |
| Ccl8 | chemokine (C-C motif) ligand 8 | −4.1 | <0.01 | 1.0 | 0.74 | −1.3 | 0.27 |
| Ccr7 | chemokine (C-C motif) receptor 7 | −4.0 | <0.05 | −1.3 | 0.27 | 1.4 | 0.22 |
| Cd3d | CD3 antigen, delta polypeptide | −3.1 | <0.05 | −1.2 | 0.17 | −1.1 | 0.37 |
| Cd68 | CD68 antigen | −2.3 | <0.05 | −1.9 | <0.05 | −1.3 | 0.52 |
| Emr1 | EGF-like module containing, mucin-like, hormone receptor-like 1 | −2.1 | <0.05 | −2.3 | <0.01 | −1.3 | 0.34 |
| Fatty acid biosynthesis and metabolism | |||||||
| Fads3 | fatty acid desaturase 3 | −2.5 | <0.01 | −4.5 | <0.001 | −1.1 | 0.72 |
| Fads1 | fatty acid desaturase 1 | 1.5 | 0.05 | −1.8 | <0.01 | −1.7 | <0.05 |
| Cidec | cell death-inducing DFFA-like effector c | −1.1 | <0.05 | −1.6 | <0.01 | −1.3 | 0.06 |
| Scd1 | stearoyl-coenzyme A desaturase 1 | −1.0 | 0.10 | −1.0 | 0.24 | 1.0 | 0.08 |
| Fasn | fatty acid synthase | −1.5 | 0.12 | −1.5 | 0.05 | 1.0 | 0.96 |
| Gpam | glycerol-3-phosphate acyltransferase, mitochondrial | 2.6 | <0.001 | 1.4 | <0.01 | 1.0 | 0.67 |
| Acss1 | acyl-CoA synthetase short-chain family member 1 | 3.2 | <0.001 | 4.9 | <0.001 | 1.1 | 0.43 |
| Acsl5 | acyl-CoA synthetase long-chain family member 5 | 3.7 | <0.001 | 4.2 | <0.001 | 1.1 | 0.43 |
| Adipokine and related effector genes | |||||||
| Lep | Leptin | −2.7 | <0.05 | −4.6 | <0.01 | −1.3 | 0.36 |
| Retn | Resistin | −1.8 | <0.001 | −2.1 | <0.001 | −1.8 | 0.05 |
| IL6st | interleukin 6 signal transducer | −1.4 | <0.001 | −1.4 | <0.01 | −1.2 | 0.07 |
| Rbp4 | retinol binding protein 4 | −1.4 | <0.05 | −2.3 | <0.001 | −1.0 | 0.84 |
| Adipoq | adiponectin, C1Q and collagen domain containing | −1.3 | <0.05 | −1.6 | <0.01 | −1.1 | 0.26 |
| Tnfaip6 | tumor necrosis factor alpha induced protein 6 | −1.3 | <0.05 | −1.2 | <0.05 | −1.0 | 0.21 |
| Adipor1 | adiponectin receptor 1 | 1.3 | <0.01 | 1.1 | <0.05 | −1.0 | 0.68 |
| Adipor2 | adiponectin receptor 2 | 1.4 | <0.05 | −1.1 | 0.20 | 1.0 | 0.83 |
The eight most highly upregulated genes in perivascular adipose tissue (PVAT) vs. subcutaneous adipose tissue (SAT) in normal diet conditions were ranked in descending order. Expression levels of the same genes in PVAT vs. visceral adipose tissue (VAT) and PVAT vs. brown adipose tissue (BAT) in normal diet conditions were then queried and are entered in the last 2 columns for comparison. Expression of genes from select categories was also queried in normal diet conditions and ranked according in descending order according to expression in SAT. FC, fold change. Significant increases in expression are bold; significant decreases in expression are italicized.