Fig. 3.

Akt upregulates miR-210. A: In H9c2 cells, there was a reduction in miR-210 response to hypoxia after treatment with Akt inhibitor (P = 0.06 compared with hypoxia alone). B: in NRCM, treatment with the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitors wortmannin at 300 nM and LY-29004 at 20 μM prevented induction of miR-210 by hypoxia (*P < 0.05 compared with hypoxia alone). C: in ARNT KO MEFs, treatment with wortmannin and LY-29004 prevented induction of miR-210 by hypoxia (*P < 0.05 and **P < 0.01 compared with hypoxia alone). D: constitutively active Akt plasmid transfection in both WT MEFs and ARNT KO MEFs induces miR-210 expression (**P < 0.01 compared with control transfection). E: in H9c2 cells, insulin treatment increased miR-210 levels (**P < 0.01 compared with no insulin treatment, n = 3 for each group). F: findings in NRCM were similar (*P < 0.05 compared with no insulin treatment, n = 3 for each group). Data are presented as means ± SE; n = 3 for each group.