Fig. 9.

EP₃ receptors in the PVN. A and B: immunoperoxidase staining for EP₃ receptors. A dotted line in A delineates the PVN. B: magnified image of EP₃ receptor immunoreactivity in the PVN. EP₃ receptor immunoreactivity was predominantly localized in neuropil but was present in a few cell bodies in the PVN. C: confocal laser-scanning microscopy of double immunofluorescence labeling for EP₃ receptor (green) and the 67-kDa form of glutamic acid decarboxylase (GAD67; red) in the PVN. This image was taken in the ventrolateral subdivision. Arrows indicate EP₃ receptor-immunoreactive terminals that contain GAD67 immunoreactivity. D: representative progress curves done in duplicate for the real-time PCR amplification of EP₃ receptor and GAPDH cDNA in the PVN and cerebral cortex at the same level. E: quantitative comparison of EP₃ receptor mRNA expression in PVN vs. cerebral cortex. Values are means ± SE and expressed as fold difference relative to cerebral cortex; n = 10 rats for each group. *P < 0.05 vs. cortex.