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Experimental protocol. A: representative time plot of the experimental protocol. B: pooled data (means ± SE) of maximum Ca2+ transient amplitude with 1 mM extracellular Ca2+ concentration ([Ca2+]o, open bar), 3 mM [Ca2+]o (black bar), or isoproterenol (ISO, gray bar). C: pooled data (means ± SE) of Ca2+ transient decline measured as the time to 50% relaxation (RT50). P < 0.05 vs. 1 mM [Ca2+]o (*) and vs. 1 and 3 mM [Ca2+]o (**) (n = 21 cells/10 hearts).
