Table 2.

Time constants for recovery from block calculated from the kinetic rates of binding and unbinding at rest compared with experimentally obtained values at −140 mV and 15°C

	Experimental Values	Calculated Values	Reference
Ventricular cells, 40 μM	650 ± 170	820 ± 110
Atrial cells, 40 μM	707 ± 160	1,020 ± 110
HEK-293 stable cell line expressing SCN5A, 30 μM	660 ± 140	845 ± 110
HEK-293 cells expressing rNav1.4-WCW, 100 μM	560 ± 40	690 ± 90	29

Values are means ± SD (in ms). Rat (r)Na_v1.4-WCW is the L435W/L437C/A438W mutant Na⁺ channel. See Table 1 for the kinetic rates of binding and unbinding at rest. HEK-293 cells, human embryonic kidney-293 cells.