Stock solution: | MN media: | DRG/SN media: |
10 mg mL-1 albumin | 12.5 μl | -- |
10 mg mL-1 apo-transferrin | 50 μl | 40 μl |
50 μg mL-1 β-estradiol | 2.7 μl | 2.2 μl |
0.1 mg mL-1 biotin | 50 μl | -- |
16 mg mL-1 catalase | 8 μl | -- |
15 mg mL-1 D- galactose | 50 μl | -- |
50 μg mL-1 hydrocortisone | 3.7 μl | 2.9 μl |
0.63 mg mL-1 progesterone | 0.5 μl | -- |
16 mg mL-1 putrescine | 50 μl | -- |
50 μg mL-1 selenium | 3.4 μl | 4.1 μl |
2.5 mg mL-1 superoxide dismutase | 50 μl | -- |
* 500 ng mL-1 nerve growth factor | -- | 1 mL |
* 100X PSN | 0.5 mL | 0.5 mL |
* B27 | 1 mL | 1 mL |
* 2 mM L-glutamine | 35 μl | 35 μl |
* Neurobasal | to 50 mL | to 50 mL |