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. 2011 Feb 15;(48):2389. doi: 10.3791/2389
Stock solution: MN media: DRG/SN media:
10 mg mL-1 albumin 12.5 μl --
10 mg mL-1 apo-transferrin 50 μl 40 μl
50 μg mL-1 β-estradiol 2.7 μl 2.2 μl
0.1 mg mL-1 biotin 50 μl --
16 mg mL-1 catalase 8 μl --
15 mg mL-1 D- galactose 50 μl --
50 μg mL-1 hydrocortisone 3.7 μl 2.9 μl
0.63 mg mL-1 progesterone 0.5 μl --
16 mg mL-1 putrescine 50 μl --
50 μg mL-1 selenium 3.4 μl 4.1 μl
2.5 mg mL-1 superoxide dismutase 50 μl --
* 500 ng mL-1 nerve growth factor -- 1 mL
* 100X PSN 0.5 mL 0.5 mL
* B27 1 mL 1 mL
* 2 mM L-glutamine 35 μl 35 μl
* Neurobasal to 50 mL to 50 mL