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. 2011 Jun 10;301(4):H1656–H1666. doi: 10.1152/ajpheart.00141.2011

Fig. 5.

Fig. 5.

The relative mRNA levels for the endogenous mouse MT1-MMP, human MT1-MMP, and the mouse latency-associated transforming growth factor-binding protein-1 (LTBP-1) were computed using real-time PCR (rtPCR) from myocardial RNA extracted from WT, MT1-MMPexp, and MT1-MMP+/− mice with no MI and at 14 days post-MI (n = 6/group). The levels were normalized to 18S mRNA, and the degree of change was computed from the relative cycle time (Ct) values obtained for the mRNA of interest and 18S mRNA. In the post-MI WT group, a significant increase in the endogenous mouse MT1-MMP mRNA levels occurred, whereas these levels were reduced in both the MT1-MMPexp and MT1-MMP+/− post-MI groups. In the MT1-MMP+/− group, relative levels were reduced from WT values with no MI as well (P < 0.05). As expected, a robust signal for human MT1-MMP mRNA was obtained in both MT1-MMPexp groups and not detected (ND) in the WT or MT1-MMP+/− groups. LTBP-1 mRNA levels were reduced in both MT1-MMPexp groups compared with WT values. In contrast, LTBP-1 mRNA levels were increased in the MT1-MMP+/− group. P < 0.05 vs. pre-MI values (#), vs. WT MI values (*), and vs. MT1-MMPexp MI values (+).