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Profile of DNA isolated from the soluble chromatin: DNA was extracted from fractionated nucleosomes obtained from chromatin derived from MNase-treated wheat nuclei and analysed on 1 % agarose gel. The lane numbers (25–39) indicate fraction numbers. Lane M: 100-bp DNA ladder. Chromatin from fractions 29 and 30 (boxed) was pooled, concentrated and used as ‘donor chromatin’ for the remodelling assay.
