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. 2011 Sep 6;108(7):1235–1246. doi: 10.1093/aob/mcr232

Fig. 4.

Fig. 4.

Nucleosome reconstitution in vitro: mononucleosomes were assembled in vitro both non-enzymatically and enzymatically as described in the Material and methods. Lane 1, mobility shift of NPS due to binding of histone octamer by the salt jump method. Lane 2, mobility shift of NPS due to ATP-dependent displacement of histone octamer in trans; Lane 3, free NPS.